Primer Design & Specificity

Design candidate primers, then screen the selected pair against one strawberry genome.

Checking Primer3

Checking ipcress

Template and assay

Template source

Paste sequence SDH gene CDS

DNA template

Template name

Assay

Product size (bp)

Pairs to return

Target region

Advanced primer constraints

Primer Tm °C

Minimum / optimum / maximum

Primer length nt

Minimum / optimum / maximum

GC content %

Specificity not checked

Check both primers against the intended genome before ordering.

Rank	Direction	Sequence (5′→3′)	Length	Tm	GC	Position	Product	Penalty	Specificity

Select a candidate pair above, or enter an existing pair.

#	Contig	Coordinates	Length	Pairing	Primer mismatches

In-silico products depend on this assembly and mismatch rule; confirm amplification experimentally.