CRISPR Guide Design
Find SpCas9 guides and inspect potential genomic matches in a selected strawberry assembly.
Checking FlashFry…
Quick start
- Choose Species and Reference assembly.
- Paste genomic DNA or use Upload FASTA: one sequence, 23–2,000 bp, file up to 12 KB. Upload a target fragment, not a whole genome. You can also use Load assembly example.
- Alternatively, select Gene ID → Locate gene → Load region, or select Genomic region, enter 1-based inclusive coordinates, then click Load region.
- Choose the mismatch limit and click Find guides. Matching is checked in the selected assembly. Click a guide ID to inspect its genomic matches; copy spacers or download results.
Target region
User ManualCandidate guides
| Reference interval | Strand | Genomic sequence (5′–3′) | MM | Gene / region overlap |
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